ZymoPURE Plasmid Miniprep Kit, 400 Preps

The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. Binding Capacity: 100 µgCulture Input: 5 mLElution Volume: 25 µl Endotoxin Levels: 1 EU/µg Equipment: Microcentrifuge and/or vacuum manifoldFormat: Spin Column Processing Time: 15 min Purity: Typical Abs 260/280 1.8 and Abs 260/230 2.0 Recommended Sample Volume: 5.0 ml Size Range: Up to 25 kb Yield: Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. As an added convenience, the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The unique spin-column design also provides zero buffer retention and a low elution volume. Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.Storage Temperature: Room TemperatureStorage Conditions: Room TemperatureApplicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. Highest Yield: Purify up to 100 µg of plasmid DNA in as little as 25 µl directly from a spin-column. coli utilized.Shipping Weight: 6.0001 lbsShipping Dimensions: 12.00 x 10.50 x 8.00Research or further manufacturing use only, not for food or drug use. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl).

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ZymoPURE Plasmid Miniprep Kit, 400 Preps